Centrifugal Partition Chromatography For CBD Purification

CBD Purification
CBD Purification
CBD Purification
CBD Purification

CBD is an active cannabinoid obtained from cannabis plants like hemp and marijuana. The compound has multiple therapeutic benefits that make it a popular medication used to handle conditions like pain, anxiety, inflammation, and more.

The US government has selectively legalized hemp-derived CBD products while the production and sale of marijuana CBD products are governed by the marijuana laws that may vary according to the state that you are in.

Being a largely unregulated market, you cannot trust every brand with its purity and quality. Your CBD experience will not go as expected if what you get is not highly pure CBD.

What Are The Challenges Of Achieving High Purity Levels Of CBD?

The complex nature of the starting materials makes chromatographic purification of CBD a quite complex process.  Traditional chromatographic purification techniques like High Performance Liquid Chromatography (HPLC) and flash chromatography that are effective in numerous applications may not adapt well to large scale purification of CBD. The primary reason is that CBD purification using HPLC and similar methods is a two-step process that requires silica resin, an expensive substance that has to be frequently replaced in the process due to the irreversible adsorption of various compounds on it.

Moreover, methods like HPLC and flash chromatography require large quantities of solvents to obtain purified natural compounds.

What Is Centrifugal Partition Chromatography?

Centrifugal Partition Chromatography (CPC) is a liquid purification technique that does not require solid supports like silica. It works based on two immiscible liquid phases; one being the mobile phase while the other being the stationary phase.

The CPC column has several stacked disks each engraved with many twin cells linked by ducts. The centrifugal field generated by the rotation of the CPC column retains the stationary phase in the column. After they are injected, the compounds that are to be purified are eluted by the mobile phase flow in the order of their partition coefficients.

What Are The Advantages Of CPC For Purifying CBD?

CPC has an upper hand to many conventional CBD purification methods. Some advantages of CPC include:

  • It is efficient in purifying complex mixtures like crude extracts of cannabis in a single step.
  • Because CPC relies on a liquid stationary phase, CPC columns do not need to be replaced unlike traditional silica-based columns used in HPLC and flash chromatography.
  • Because the CPC column can be loaded with many solvents, it contributes to cost reduction.

CBD products introduced into the market by fraudster manufacturers may contain unwanted substances like metal toxins and harmful chemicals that the hemp crops may have accumulated from the soil. Check the third party lab results of the product to ensure that it is free of any toxic chemicals or microbes before buying a product. Take a look here for some of the most pure CBD products.